Английская Википедия:ADA (buffer)

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Версия от 00:14, 27 декабря 2023; EducationBot (обсуждение | вклад) (Новая страница: «{{Английская Википедия/Панель перехода}} {{chembox | Name = ADA | ImageFile = ADA (buffer).png | ImageName = Chemical structure of ADA | SystematicName = 2,2′-[(2-amino-2-oxoethyl)azanediyl]diacetic acid | OtherNames = ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid |Section1={{Chembox Identifiers | InChI = 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13) | InChIKe...»)
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ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H+, Ca2+, and Mg2+, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.[1]

References

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