Английская Википедия:Bismuth sulfite agar

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Версия от 19:54, 9 февраля 2024; EducationBot (обсуждение | вклад) (Новая страница: «{{Английская Википедия/Панель перехода}} thumb|300px|After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). '''Bismuth sulfite agar''' is a type of agar media used to isolate ''Sal...»)
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Файл:Agarplates.jpg
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars).

Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. Bismuth and brilliant green (dye) both inhibit gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.

Bismuth sulfite agar typically contains (w/v):[1]

1.6% bismuth sulfite Bi2(SO3)3
1.0% pancreatic digest of casein
1.0% pancreatic digest of animal tissue
1.0% beef extract
1.0% glucose
0.8% dibasic sodium phosphate
0.06% ferrous sulfate • 7 water
pH adjusted to 7.7 at 25 °C

This medium is boiled for sterility, not autoclaved.

References

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Шаблон:Growth media


Шаблон:Microbiology-stub