Chromosome 10 open reading frame 35 (c10orf35) is a gene that in humans, encodes for a protein-binding,[1]transmembrane[2] protein. The protein contains the domain of unknown function 4605 (DUF4605)[1] which belongs to the protein family pfam15378.[3] This gene is located at locus 10q22.1.[1]
The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal,[4] and the isoelectric point was predicted to be 11.5.[4] The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal.[4] The isoelectric point was predicted to be 6.9,[4] which is significantly less basic than the full protein.
Properties and structure
This protein includes a highly conserved region between amino acids 1 and 19 discovered by MSA.[5] DUF 4605 is located between amino acids 62 and 92.[1]
The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5,[6] an α-helix between 61 and 72,[6] and a transmembrane α-helix between 92 and 112.[7]
The serine in position 62 can be phosphorylated to form a phosphoserine group.[7]
Homology
Paralogs
The protein of interest has a similar, paralogous domain found in c4orf32,[8] which is conserved in mammals, birds, very few reptiles, and one amphibian.[9]
Orthologs
The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate.[10] Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region were confirmed.[5]
The c10orf35 protein has expression over the 75th percentile in the brain, spinal cord, and male reproductive organs.[12] Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis.[13] In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain.[13]