Английская Википедия:Dilution cloning
Dilution cloning or cloning by limiting dilution [1][2] describes a procedure to obtain a monoclonal cell population starting from a polyclonal mass of cells. This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made. Appropriate dilutions are then made, depending on cell number in the starting population, as well as the viability and characteristics of the cells being cloned. After the final dilutions are produced, aliquots of the suspension are plated[1] or placed in wells[2] and incubated. If all works correctly, a monoclonal cell colony will be produced. Applications for the procedure include cloning of parasites,[3] T cells,[4] transgenic cells,[5] macrophages.[6] and hematopoietic stem cells.[7]
References
External links
- Robert Staszewski "Cloning by Limiting Dilution: an improved estimate that an interesting culture is monoclonal" [1]
- John A. Ryan "Cell Cloning by Serial Dilution in 96 Well Plates".
- Vilma Maldonado,Jorge Meléndez-Zajgla "A modified method for cloning adherent mammalian cells".
- "Cloning by Limiting Dilution".Шаблон:Dead link
- "Cloning by limiting dilution".
- Nanci Donacki "Cloning by Limiting Dilution of Hybridoma".