Английская Википедия:AE1/AE3

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Файл:HE histopathology and CK AE1-AE3 immunohistochemistry in endometrial adenocarcinoma metastasis to lymph node.png
Histopathology of a pelvic lymph node in a patient with endometrial adenocarcinoma (FIGO grade 1):
- Left panel shows H&E staining and low magnification, where any presence of small metastases is hard to see.
- Middle panel shows immunohistochemistry for CK AE1/AE3, which highlights even small tumor nests.
- The right panel shows high magnification on a positive area, confirming adenocarcinoma, as it shows tumor cells with large nuclei and prominent nucleoli.

AE1/AE3 is an antibody cocktail that is used in immunohistochemistry, being generally positive in the cytoplasm of carcinomas (cancers of epithelial origin).[1][2]

Targets

The antibody cocktail binds to cytokeratin 1 - 8, 10, 14 - 16 and 19 (but not CK17 or CK18).[1] It is therefore used as a marker of carcinomas, such as depth of invasion and metastases.[1] For example, it is both relatively sensitive and specific for detection of breast cancer metastasis to sentinel lymph nodes.[3]

It may cross-react with GFAP, leading to aberrant staining of glial tumors such as ependymoma, glioblastoma and schwannoma.[1] It may also stain myofibroblasts and smooth muscle cells.[1] Furthermore, it may stain nodal epithelial cells that has contaminated a tumor from recent biopsy.[1]

See also

References

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Шаблон:Pathology-stub

  1. 1,0 1,1 1,2 1,3 1,4 1,5 Шаблон:Cite web Revised: 26 November 2019
  2. Cytoplasmic stain: Шаблон:Cite web
  3. Шаблон:Cite journal