Шаблон:Short descriptionШаблон:ChemboxШаблон:PH indicator templateBromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins.[1] It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations. In clinical practise, it is commonly used as a diagnostic technique. The most common use of bromocresol green is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. In chemistry, bromocresol green is used in Thin-layer chromatography staining solutions to visualize acidic compounds.
Файл:Bromocresolo.jpgFrom left to right solutions of 0.1 M HCl, 3 buffer solutions of pH 3.78, 3 of pH 4.00, 3 of pH 4.62 and NaOH 0.1 M after adding different amounts of bromocresol green (more in darker solutions)
The acid dissociation constant (pKa) of this reaction is 4.8.[3] Tap water is sufficiently basic to give a solution of bromocresol green its characteristic blue-green color.[4]
Файл:Bromocresol green spectrum.pngAbsorbance spectrum of bromocresol green at different pH values. The isosbestic point occurs where the acid and basic forms and mixtures thereof have the same absorbance
The acid and basic forms of this dye have an isosbestic point in their UV-Visible spectrum, around 515 nm, indicate that the two forms interconvert directly without forming any other substance.
An ethanol solution (0.04 wt%) of bromocresol green has been proposed for TLC staining and is suitable for visualisation of the compounds with functional groups whose pKa is below 5.0 (carboxylic acids, sulfonic acids, etc.). These appear as yellow spots on a light or dark blue background; no heating is necessary. Bromophenol blue solution can be used for the same purpose.
The compound is synthesized by bromination of cresol purple (m-cresolsulfonphthalein).
Uses
It is used as a pH indicator and as a tracking dye for DNAagarose gel electrophoresis. It can be used in its free acid form (light brown solid), or as a sodiumsalt (dark green solid). It is also an inhibitor of the prostaglandin E2 transport protein. Additional applications include use in sol-gel matrices,[5] the detection of ammonia,[6] and the measurement of albumin in human plasma and serum.[7]
Safety
Bromocresol green may cause irritation. Skin and eye contact should be avoided.
↑Anonymous. Bromocresol Green. In The Merck index : an encyclopedia of chemicals, drugs, and biologicals; Windholz, M., Ed.; Merck & Co., Inc.: Rahway, N.J., 1983; pp 191.
