Английская Википедия:Cholesterol oxidase

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Шаблон:Short description Шаблон:Infobox enzyme Шаблон:Infobox protein family In enzymology, a cholesterol oxidase (Шаблон:EC number) is an enzyme that catalyzes the chemical reaction

cholesterol + O₂ <math>\rightleftharpoons</math> cholest-4-en-3-one + H₂O₂

Thus, the two substrates of this enzyme are cholesterol and O₂, whereas its two products are cholest-4-en-3-one and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one.

Structural studies

As of late 2007, 14 structures have been solved for this class of enzymes, with PDB accession codes Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, Шаблон:PDB link, and Шаблон:PDB link.

Further reading

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Шаблон:1.1-enzyme-stub