Английская Википедия:Glypican 3

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Шаблон:Short description Шаблон:Cs1 config Шаблон:Infobox gene Glypican-3 is a protein that, in humans, is encoded by the GPC3 gene.[1][2][3][4] The GPC3 gene is located on human X chromosome (Xq26) where the most common gene (Isoform 2, GenBank Accession No.: NP_004475) encodes a 70-kDa core protein with 580 amino acids.[5] Three variants have been detected that encode alternatively spliced forms termed Isoforms 1 (NP_001158089), Isoform 3 (NP_001158090) and Isoform 4 (NP_001158091).[5]

Structure and function

Файл:Schematic of the glypican-3 protein..tif
Schematic of the glypican-3 (GPC3) protein[5]

The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.[5] Six glypicans (GPC1-6) have been identified in mammals. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.[3] GPC3 has been found to regulate Wnt/β-catenin and Yap signaling pathways.[5][6][7][8][9][10][11][12] GPC3 interacts with both Wnt and frizzled (FZD) to form a complex and triggers downstream signaling.[7][13] The core protein of GPC3 may serve as a co-receptor or a receiver for Wnt. A cysteine-rich domain at the N-lobe of GPC3 has been identified as a hydrophobic groove that interacts with Wnt3a.[13] Blocking the Wnt binding domain on GPC3 using the HN3 single domain antibody can inhibit Wnt activation.[13] Wnt also recognizes a heparan sulfate structure on GPC3 , which contains IdoA2S and GlcNS6S, and that the 3-O-sulfation in GlcNS6S3S significantly enhances the binding of Wnt to heparan sulfate.[6] GPC3 also modulates Yap signaling.[8] It might interact with FAT1 on the cell surface.[11]

Disease linkage

Deletion mutations in this gene are associated with Simpson–Golabi–Behmel syndrome.[1]

Diagnostic utility

Glypican 3 immunostaining has utility for differentiating hepatocellular carcinoma (HCC)[14] and dysplastic changes in cirrhotic livers; HCC stains with glypican 3, while liver with dysplastic changes and/or cirrhotic changes does not.[15] Using the YP7 murine monoclonal antibody, GPC3 protein expression is found in HCC, not in normal liver and cholangiocarcinoma.[16] The YP7 murine antibody has been humanized and named as 'hYP7'.[17] GPC3 is also expressed to a lesser degree in melanoma, ovarian clear-cell carcinomas, yolk sac tumors, neuroblastoma, hepatoblastoma, Wilms' tumor cells, and other tumors.[5] However, the significance of GPC3 as a diagnostic tool for human tumors other than HCC is unclear.Шаблон:Citation needed

Therapeutic potential

To validate GPC3 as a therapeutic target in liver cancer, the anti-GPC3 therapeutic antibodies GC33,[18] YP7,[16] HN3[8] and HS20[9][19] have been made and widely tested. The laboratory of Dr. Mitchell Ho at the National Cancer Institute, NIH (Bethesda, Maryland, US) has generated YP7 and other murine monoclonal antibodies that recognize the C-lobe of GPC3 by hybridoma technology.[16] These antibodies have been humanized (e.g. hYP7) via antibody engineering for clinical applications.[17] The Ho lab has also identified the human single-domain antibody ('human nanobody') HN3[8] targeting the N-lobe of GPC3 [13] and the human monoclonal antibody HS20[9][20] targeting the heparan sulfate chains on GPC3 by phage display technology. Both HN3 and HS20 antibodies inhibit Wnt signaling in liver cancer cells . The immunotoxins based on HN3,[10][21][22] the antibody-drug conjugates based on hYP7[23] and the T-cell engaging bispecific antibodies derived from YP7[24][25] and GC33,[26] have been developed for treating liver cancer. The chimeric antigen receptor (CAR) T cell immunotherapies based on GC33,[27] hYP7[28][29] and HN3[30] are being developed at various stages for treating liver cancer. In mice with xenograft or orthoptic liver tumors, CAR (hYP7) T cells can eliminate GPC3-positive cancer cells, by inducing perforin- and granzyme-mediated cell death and reducing Wnt signaling in tumor cells.[29] CAR (hYP7) T cells are being evaluated at a clinical trial (NCT05003895) at the NIH.

See also

References

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Further reading

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External links

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  1. ↑ 1,0 1,1 Шаблон:Cite journal
  2. ↑ Шаблон:Cite journal
  3. ↑ 3,0 3,1 Шаблон:Cite web
  4. ↑ Шаблон:Cite journal
  5. ↑ 5,0 5,1 5,2 5,3 5,4 5,5 Шаблон:Cite journal
  6. ↑ 6,0 6,1 Шаблон:Cite journal
  7. ↑ 7,0 7,1 Шаблон:Cite journal
  8. ↑ 8,0 8,1 8,2 8,3 Шаблон:Cite journal
  9. ↑ 9,0 9,1 9,2 Шаблон:Cite journal
  10. ↑ 10,0 10,1 Шаблон:Cite journal
  11. ↑ 11,0 11,1 Шаблон:Cite journal
  12. ↑ Шаблон:Cite journal
  13. ↑ 13,0 13,1 13,2 13,3 Шаблон:Cite journal
  14. ↑ Шаблон:Cite journal
  15. ↑ Шаблон:Cite journal
  16. ↑ 16,0 16,1 16,2 Шаблон:Cite journal
  17. ↑ 17,0 17,1 Шаблон:Cite journal
  18. ↑ Шаблон:Cite journal
  19. ↑ Шаблон:Cite journal
  20. ↑ Шаблон:Cite journal
  21. ↑ Шаблон:Cite journal
  22. ↑ Шаблон:Cite journal
  23. ↑ Шаблон:Cite journal
  24. ↑ Шаблон:Cite web
  25. ↑ Шаблон:Cite journal
  26. ↑ Шаблон:Cite journal
  27. ↑ Шаблон:Cite journal
  28. ↑ Шаблон:Cite journal
  29. ↑ 29,0 29,1 Шаблон:Cite journal
  30. ↑ Шаблон:Cite journal
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